Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in Arabidopsis thaliana.
Identifieur interne : 000A62 ( Main/Exploration ); précédent : 000A61; suivant : 000A63Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in Arabidopsis thaliana.
Auteurs : Muhammad Shahid [Corée du Sud] ; Qari Muhammad Imran [Corée du Sud] ; Adil Hussain [Pakistan] ; Murtaza Khan [Corée du Sud] ; Sang Uk Lee [Corée du Sud] ; Bong Gyu Mun [Corée du Sud] ; Byung-Wook Yun [Corée du Sud]Source :
- Genes [ 2073-4425 ] ; 2019.
Descripteurs français
- KwdFr :
- Arabidopsis (génétique), Arabidopsis (métabolisme), Cellules souches (effets des médicaments et des substances chimiques), Cellules souches (métabolisme), Cellules végétales (effets des médicaments et des substances chimiques), Cellules végétales (métabolisme), Cystéine (analogues et dérivés), Cystéine (pharmacologie), Donneur d'oxyde nitrique (pharmacologie), Gènes de plante (MeSH), Monoxyde d'azote (métabolisme), Protein-Serine-Threonine Kinases (génétique), Protein-Serine-Threonine Kinases (métabolisme), Protéines d'Arabidopsis (génétique), Protéines d'Arabidopsis (métabolisme), Régions promotrices (génétique) (MeSH), Régulation de l'expression des gènes végétaux (MeSH), S-Nitrosothiols (pharmacologie), Transcriptome (MeSH).
- MESH :
- analogues et dérivés : Cystéine.
- effets des médicaments et des substances chimiques : Cellules souches, Cellules végétales.
- génétique : Arabidopsis, Protein-Serine-Threonine Kinases, Protéines d'Arabidopsis.
- métabolisme : Arabidopsis, Cellules souches, Cellules végétales, Monoxyde d'azote, Protein-Serine-Threonine Kinases, Protéines d'Arabidopsis.
- pharmacologie : Cystéine, Donneur d'oxyde nitrique, S-Nitrosothiols.
- Gènes de plante, Régions promotrices (génétique), Régulation de l'expression des gènes végétaux, Transcriptome.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Arabidopsis Proteins (genetics), Arabidopsis Proteins (metabolism), Cysteine (analogs & derivatives), Cysteine (pharmacology), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Nitric Oxide (metabolism), Nitric Oxide Donors (pharmacology), Plant Cells (drug effects), Plant Cells (metabolism), Promoter Regions, Genetic (MeSH), Protein-Serine-Threonine Kinases (genetics), Protein-Serine-Threonine Kinases (metabolism), S-Nitrosothiols (pharmacology), Stem Cells (drug effects), Stem Cells (metabolism), Transcriptome (MeSH).
- MESH :
- chemical , analogs & derivatives : Cysteine.
- chemical , genetics : Arabidopsis Proteins, Protein-Serine-Threonine Kinases.
- drug effects : Plant Cells, Stem Cells.
- genetics : Arabidopsis.
- metabolism : Arabidopsis, Arabidopsis Proteins, Nitric Oxide, Plant Cells, Protein-Serine-Threonine Kinases, Stem Cells.
- chemical , pharmacology : Cysteine, Nitric Oxide Donors, S-Nitrosothiols.
- Gene Expression Regulation, Plant, Genes, Plant, Promoter Regions, Genetic, Transcriptome.
Abstract
Plant stem cells are pluripotent cells that have diverse applications in regenerative biology and medicine. However, their roles in plant growth and disease resistance are often overlooked. Using high-throughput RNA-seq data, we identified approximately 20 stem cell-related differentially expressed genes (DEGs) that were responsive to the nitric oxide (NO) donor S-nitrosocysteine (CySNO) after six hours of infiltration. Among these DEGs, the highest number of positive correlations (R ≥ 0.8) was observed for CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) 12. Gene ontology (GO) terms for molecular function showed DEGs associated with signal transduction and receptor activity. A promoter study of these DEGs showed the presence of cis-acting elements that are involved in growth as well as the regulation of abiotic and biotic stress. Phylogenetic analysis of the Arabidopsis stem cell-related genes and their common orthologs in rice, soybean, poplar, and tomato suggested that most soybean stem cell-related genes were grouped with the Arabidopsis CLE type of stem cell genes, while the rice stem cell-related genes were grouped with the Arabidopsis receptor-like proteins. The functional genomic-based characterization of the role of stem cell DEGs showed that under control conditions, the clv1 mutant showed a similar phenotype to that of the wild-type (WT) plants; however, under CySNO-mediated nitrosative stress, clv1 showed increased shoot and root length compared to WT. Furthermore, the inoculation of clv1 with virulent Pst DC3000 showed a resistant phenotype with fewer pathogens growing at early time points. The qRT-PCR validation and correlation with the RNA-seq data showed a Pearson correlation coefficient of >0.8, indicating the significantly high reliability of the RNA-seq analysis.
DOI: 10.3390/genes10020173
PubMed: 30813477
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in <i>Arabidopsis thaliana</i>.</title><author><name sortKey="Shahid, Muhammad" sort="Shahid, Muhammad" uniqKey="Shahid M" first="Muhammad" last="Shahid">Muhammad Shahid</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. shahidariswat@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Imran, Qari Muhammad" sort="Imran, Qari Muhammad" uniqKey="Imran Q" first="Qari Muhammad" last="Imran">Qari Muhammad Imran</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mimranbot@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Hussain, Adil" sort="Hussain, Adil" uniqKey="Hussain A" first="Adil" last="Hussain">Adil Hussain</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture Abdul Wali Khan University, Mardan 23200, Pakistan. adilhussain@awkum.edu.pk.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>Department of Agriculture Abdul Wali Khan University, Mardan 23200</wicri:regionArea><wicri:noRegion>Mardan 23200</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Murtaza" sort="Khan, Murtaza" uniqKey="Khan M" first="Murtaza" last="Khan">Murtaza Khan</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. murtazakhan.bio@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Uk" sort="Lee, Sang Uk" uniqKey="Lee S" first="Sang Uk" last="Lee">Sang Uk Lee</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. uk0gam@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Mun, Bong Gyu" sort="Mun, Bong Gyu" uniqKey="Mun B" first="Bong Gyu" last="Mun">Bong Gyu Mun</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mun0301@naver.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Yun, Byung Wook" sort="Yun, Byung Wook" uniqKey="Yun B" first="Byung-Wook" last="Yun">Byung-Wook Yun</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. bwyun@knu.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30813477</idno><idno type="pmid">30813477</idno><idno type="doi">10.3390/genes10020173</idno><idno type="wicri:Area/Main/Corpus">000A12</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A12</idno><idno type="wicri:Area/Main/Curation">000A12</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A12</idno><idno type="wicri:Area/Main/Exploration">000A12</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in <i>Arabidopsis thaliana</i>.</title><author><name sortKey="Shahid, Muhammad" sort="Shahid, Muhammad" uniqKey="Shahid M" first="Muhammad" last="Shahid">Muhammad Shahid</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. shahidariswat@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Imran, Qari Muhammad" sort="Imran, Qari Muhammad" uniqKey="Imran Q" first="Qari Muhammad" last="Imran">Qari Muhammad Imran</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mimranbot@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Hussain, Adil" sort="Hussain, Adil" uniqKey="Hussain A" first="Adil" last="Hussain">Adil Hussain</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agriculture Abdul Wali Khan University, Mardan 23200, Pakistan. adilhussain@awkum.edu.pk.</nlm:affiliation><country xml:lang="fr">Pakistan</country><wicri:regionArea>Department of Agriculture Abdul Wali Khan University, Mardan 23200</wicri:regionArea><wicri:noRegion>Mardan 23200</wicri:noRegion></affiliation></author><author><name sortKey="Khan, Murtaza" sort="Khan, Murtaza" uniqKey="Khan M" first="Murtaza" last="Khan">Murtaza Khan</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. murtazakhan.bio@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Sang Uk" sort="Lee, Sang Uk" uniqKey="Lee S" first="Sang Uk" last="Lee">Sang Uk Lee</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. uk0gam@gmail.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Mun, Bong Gyu" sort="Mun, Bong Gyu" uniqKey="Mun B" first="Bong Gyu" last="Mun">Bong Gyu Mun</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mun0301@naver.com.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author><author><name sortKey="Yun, Byung Wook" sort="Yun, Byung Wook" uniqKey="Yun B" first="Byung-Wook" last="Yun">Byung-Wook Yun</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. bwyun@knu.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566</wicri:regionArea><wicri:noRegion>Daegu 41566</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genes</title><idno type="ISSN">2073-4425</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Arabidopsis Proteins (genetics)</term><term>Arabidopsis Proteins (metabolism)</term><term>Cysteine (analogs & derivatives)</term><term>Cysteine (pharmacology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Nitric Oxide (metabolism)</term><term>Nitric Oxide Donors (pharmacology)</term><term>Plant Cells (drug effects)</term><term>Plant Cells (metabolism)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Protein-Serine-Threonine Kinases (genetics)</term><term>Protein-Serine-Threonine Kinases (metabolism)</term><term>S-Nitrosothiols (pharmacology)</term><term>Stem Cells (drug effects)</term><term>Stem Cells (metabolism)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Cellules souches (effets des médicaments et des substances chimiques)</term><term>Cellules souches (métabolisme)</term><term>Cellules végétales (effets des médicaments et des substances chimiques)</term><term>Cellules végétales (métabolisme)</term><term>Cystéine (analogues et dérivés)</term><term>Cystéine (pharmacologie)</term><term>Donneur d'oxyde nitrique (pharmacologie)</term><term>Gènes de plante (MeSH)</term><term>Monoxyde d'azote (métabolisme)</term><term>Protein-Serine-Threonine Kinases (génétique)</term><term>Protein-Serine-Threonine Kinases (métabolisme)</term><term>Protéines d'Arabidopsis (génétique)</term><term>Protéines d'Arabidopsis (métabolisme)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>S-Nitrosothiols (pharmacologie)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Cysteine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Arabidopsis Proteins</term><term>Protein-Serine-Threonine Kinases</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Cystéine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plant Cells</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Cellules souches</term><term>Cellules végétales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Protein-Serine-Threonine Kinases</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Arabidopsis Proteins</term><term>Nitric Oxide</term><term>Plant Cells</term><term>Protein-Serine-Threonine Kinases</term><term>Stem Cells</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Arabidopsis</term><term>Cellules souches</term><term>Cellules végétales</term><term>Monoxyde d'azote</term><term>Protein-Serine-Threonine Kinases</term><term>Protéines d'Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Cystéine</term><term>Donneur d'oxyde nitrique</term><term>S-Nitrosothiols</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cysteine</term><term>Nitric Oxide Donors</term><term>S-Nitrosothiols</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Promoter Regions, Genetic</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes de plante</term><term>Régions promotrices (génétique)</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Plant stem cells are pluripotent cells that have diverse applications in regenerative biology and medicine. However, their roles in plant growth and disease resistance are often overlooked. Using high-throughput RNA-seq data, we identified approximately 20 stem cell-related differentially expressed genes (DEGs) that were responsive to the nitric oxide (NO) donor S-nitrosocysteine (CySNO) after six hours of infiltration. Among these DEGs, the highest number of positive correlations (<i>R</i> ≥ 0.8) was observed for <i>CLAVATA3</i>/<i>EMBRYO SURROUNDING REGION-RELATED</i> (<i>CLE</i>) 12. Gene ontology (GO) terms for molecular function showed DEGs associated with signal transduction and receptor activity. A promoter study of these DEGs showed the presence of <i>cis</i>-acting elements that are involved in growth as well as the regulation of abiotic and biotic stress. Phylogenetic analysis of the <i>Arabidopsis</i> stem cell-related genes and their common orthologs in rice, soybean, poplar, and tomato suggested that most soybean stem cell-related genes were grouped with the <i>Arabidopsis</i> CLE type of stem cell genes, while the rice stem cell-related genes were grouped with the <i>Arabidopsis</i> receptor-like proteins. The functional genomic-based characterization of the role of stem cell DEGs showed that under control conditions, the <i>clv1</i> mutant showed a similar phenotype to that of the wild-type (WT) plants; however, under CySNO-mediated nitrosative stress, <i>clv1</i> showed increased shoot and root length compared to WT. Furthermore, the inoculation of <i>clv1</i> with virulent <i>Pst</i> DC3000 showed a resistant phenotype with fewer pathogens growing at early time points. The qRT-PCR validation and correlation with the RNA-seq data showed a Pearson correlation coefficient of >0.8, indicating the significantly high reliability of the RNA-seq analysis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30813477</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>23</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2073-4425</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><Issue>2</Issue><PubDate><Year>2019</Year><Month>02</Month><Day>24</Day></PubDate></JournalIssue><Title>Genes</Title><ISOAbbreviation>Genes (Basel)</ISOAbbreviation></Journal><ArticleTitle>Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in <i>Arabidopsis thaliana</i>.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E173</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/genes10020173</ELocationID><Abstract><AbstractText>Plant stem cells are pluripotent cells that have diverse applications in regenerative biology and medicine. However, their roles in plant growth and disease resistance are often overlooked. Using high-throughput RNA-seq data, we identified approximately 20 stem cell-related differentially expressed genes (DEGs) that were responsive to the nitric oxide (NO) donor S-nitrosocysteine (CySNO) after six hours of infiltration. Among these DEGs, the highest number of positive correlations (<i>R</i> ≥ 0.8) was observed for <i>CLAVATA3</i>/<i>EMBRYO SURROUNDING REGION-RELATED</i> (<i>CLE</i>) 12. Gene ontology (GO) terms for molecular function showed DEGs associated with signal transduction and receptor activity. A promoter study of these DEGs showed the presence of <i>cis</i>-acting elements that are involved in growth as well as the regulation of abiotic and biotic stress. Phylogenetic analysis of the <i>Arabidopsis</i> stem cell-related genes and their common orthologs in rice, soybean, poplar, and tomato suggested that most soybean stem cell-related genes were grouped with the <i>Arabidopsis</i> CLE type of stem cell genes, while the rice stem cell-related genes were grouped with the <i>Arabidopsis</i> receptor-like proteins. The functional genomic-based characterization of the role of stem cell DEGs showed that under control conditions, the <i>clv1</i> mutant showed a similar phenotype to that of the wild-type (WT) plants; however, under CySNO-mediated nitrosative stress, <i>clv1</i> showed increased shoot and root length compared to WT. Furthermore, the inoculation of <i>clv1</i> with virulent <i>Pst</i> DC3000 showed a resistant phenotype with fewer pathogens growing at early time points. The qRT-PCR validation and correlation with the RNA-seq data showed a Pearson correlation coefficient of >0.8, indicating the significantly high reliability of the RNA-seq analysis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shahid</LastName><ForeName>Muhammad</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. shahidariswat@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Imran</LastName><ForeName>Qari Muhammad</ForeName><Initials>QM</Initials><Identifier Source="ORCID">0000-0002-7072-451X</Identifier><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mimranbot@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hussain</LastName><ForeName>Adil</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-7803-429X</Identifier><AffiliationInfo><Affiliation>Department of Agriculture Abdul Wali Khan University, Mardan 23200, Pakistan. adilhussain@awkum.edu.pk.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Khan</LastName><ForeName>Murtaza</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. murtazakhan.bio@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Sang Uk</ForeName><Initials>SU</Initials><Identifier Source="ORCID">0000-0002-6276-9662</Identifier><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. uk0gam@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mun</LastName><ForeName>Bong Gyu</ForeName><Initials>BG</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. mun0301@naver.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yun</LastName><ForeName>Byung-Wook</ForeName><Initials>BW</Initials><Identifier Source="ORCID">0000-0002-5451-2365</Identifier><AffiliationInfo><Affiliation>Laboratory of Plant Functional Genomics, School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea. bwyun@knu.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>02</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Genes (Basel)</MedlineTA><NlmUniqueID>101551097</NlmUniqueID><ISSNLinking>2073-4425</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029681">Arabidopsis Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020030">Nitric Oxide Donors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026403">S-Nitrosothiols</NameOfSubstance></Chemical><Chemical><RegistryNumber>31C4KY9ESH</RegistryNumber><NameOfSubstance UI="D009569">Nitric Oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>926P2322P4</RegistryNumber><NameOfSubstance UI="C021315">S-nitrosocysteine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="C118166">CLV1 protein, Arabidopsis</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.11.1</RegistryNumber><NameOfSubstance UI="D017346">Protein-Serine-Threonine Kinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>K848JZ4886</RegistryNumber><NameOfSubstance UI="D003545">Cysteine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029681" MajorTopicYN="N">Arabidopsis Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003545" MajorTopicYN="N">Cysteine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009569" MajorTopicYN="N">Nitric Oxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020030" MajorTopicYN="N">Nitric Oxide Donors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059828" MajorTopicYN="N">Plant Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017346" MajorTopicYN="N">Protein-Serine-Threonine Kinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D026403" MajorTopicYN="N">S-Nitrosothiols</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013234" MajorTopicYN="N">Stem Cells</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Arabidopsis thaliana</Keyword><Keyword MajorTopicYN="Y">basal defense</Keyword><Keyword MajorTopicYN="Y">gene ontology</Keyword><Keyword MajorTopicYN="Y">promoter analysis</Keyword><Keyword MajorTopicYN="Y">stem cells</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30813477</ArticleId><ArticleId IdType="pii">genes10020173</ArticleId><ArticleId IdType="doi">10.3390/genes10020173</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>Pakistan</li></country></list><tree><country name="Corée du Sud"><noRegion><name sortKey="Shahid, Muhammad" sort="Shahid, Muhammad" uniqKey="Shahid M" first="Muhammad" last="Shahid">Muhammad Shahid</name></noRegion><name sortKey="Imran, Qari Muhammad" sort="Imran, Qari Muhammad" uniqKey="Imran Q" first="Qari Muhammad" last="Imran">Qari Muhammad Imran</name><name sortKey="Khan, Murtaza" sort="Khan, Murtaza" uniqKey="Khan M" first="Murtaza" last="Khan">Murtaza Khan</name><name sortKey="Lee, Sang Uk" sort="Lee, Sang Uk" uniqKey="Lee S" first="Sang Uk" last="Lee">Sang Uk Lee</name><name sortKey="Mun, Bong Gyu" sort="Mun, Bong Gyu" uniqKey="Mun B" first="Bong Gyu" last="Mun">Bong Gyu Mun</name><name sortKey="Yun, Byung Wook" sort="Yun, Byung Wook" uniqKey="Yun B" first="Byung-Wook" last="Yun">Byung-Wook Yun</name></country><country name="Pakistan"><noRegion><name sortKey="Hussain, Adil" sort="Hussain, Adil" uniqKey="Hussain A" first="Adil" last="Hussain">Adil Hussain</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000A62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30813477
|texte= Comprehensive Analyses of Nitric Oxide-Induced Plant Stem Cell-Related Genes in Arabidopsis thaliana.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30813477" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |